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KMID : 0381919980280030299
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Korean Journal of Microscopy
1998 Volume.28 No. 3 p.299 ~ p.306
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Tissue Preparation with t-Butyl Alcohol Freeze-drying Method for Scanning Electron Microscopy: Application for Rat Liver
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Uhm Chang-Sub
Park Eun-Kyung
Park Chang-Hyun
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Abstract
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T-butyl alcohol (TBA) freeze-drying method originally designed by Inoue and Osadake (1989) was adopted to dry specimens for scanning electron microscopy and the results were compared with those dried using critical point dryer (CPD). Small pieces $(1\times1\times3mm)$ of liver of Sprague-Dawley rats were cut and fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer after anesthesia, and processed for scanning electron microscopy by several modifications of TBA freeze-drying methods and by the standard preparation method using CPD. The bile canaliculi and sinusoidal endothelial surface were observed. Tissue dehydrated with TBA before TBA freeze-drying preserved the structures best comparable to those prepared with CPD. This result suggests that combination of dehydration with TBA and TBA freeze-drying is a superior method to the original TBA freeze-drying method dehydrated with ethanol.
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KEYWORD
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Bile canaliculi, Scanning electron microscopy, T-butyl alcohol freeze-drying method, Tissue preparation
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